MACS

Magnetic Activated Cell Sorting (MACS) is an advanced sperm selection technique used in assisted reproductive treatments like IVF and ICSI. It is particularly useful for eliminating apoptotic (damaged or dying) sperm cells, which may negatively affect fertilization, embryo quality, and pregnancy outcomes.

MACS is based on the biological principle of apoptosis—a natural process where sperm cells destined to die undergo membrane changes. One such change is the externalization of Phosphatidylserine (PS) on the sperm cell surface. This acts as an early marker of apoptosis and is detected using a protein called Annexin V.

The MACS Technique and Its Clinical Applications

How MACS works:

• Annexin V, a protein that binds to phosphatidylserine, is tagged with paramagnetic beads.
• When a sperm sample is passed through a magnetic column, apoptotic sperm (Annexin V-positive) bind to the column and are retained.
• Healthy, non-apoptotic sperm (Annexin V-negative) pass through and are collected for use in IVF or ICSI.
• This helps in enriching the sperm population with genetically healthier cells.

Indications for Using MACS:

• More than two failed IVF or ICSI cycles
• High Sperm DNA Fragmentation Index (DFI)
• Use of frozen sperm samples in ICSI
• Teratozoospermia (abnormal sperm morphology)
• Paternal age above 50 years

Benefits of MACS:

• Improved fertilization rates
• Better embryo quality and blastocyst formation
• Reduced miscarriage rates
• Higher chances of pregnancy in repeated IVF failures
• Selection of genetically competent sperm

At Dr. Suparna’s IVF Clinic, we utilize MACS technology to offer better outcomes for couples facing complex fertility challenges. By selecting non-apoptotic sperm, MACS helps in achieving better embryo development and higher implantation rates, especially for patients with previous failed IVF attempts or known male factor infertility.

If you have experienced unsuccessful fertility treatments or have been diagnosed with high sperm DNA damage, MACS may significantly improve your chances of success.